Wealdstone have sacked manager Sam Cox after eight months in charge following three successive National League defeats.
Cox, 35, who appeared in season three of the Apple TV series Ted Lasso, was in his second spell as manager, having previously served in an interim capacity three seasons ago when he helped the Stones avoid relegation.
First-team coach Danny Payne will take charge for Tuesday's home match against Brackley Town.
"With so many games still left to play this season, our shared feeling was that a new energy and fresh impetus is urgently required to re-focus everyone's minds on both the league and cup challenges ahead of us," Wealdstone chairman Rory Fitzgerald told the club website.
"Like all teams, we've had our injury challenges along the way, but we believe our squad can get back to the early-season form that would both secure our National League status and also give us a great chance of reaching Wembley."
Wealdstone travel to Kidderminster on Saturday in the sixth round of the FA Trophy.
The recent slump, which included a 3-0 loss to Sutton on Saturday, dropped the Stones to 17th in the table and within six points of the relegation zone.